Single-Cell RNA Sequencing Maps Immune Cell Heterogeneity in Mice with Allogeneic Cardiac Transplantation

Objective: Immune cells play important roles in mediating allograft rejection and tolerance after cardiac transplantation. However, immune cell heterogeneity at the single-cell level, how states shape transplantation immunity, remain incompletely characterized. Methods: We performed RNA sequencing (scRNA-seq) on LNs from a mouse syngeneic allogeneic model. Nine T clusters were identified through unsupervised analysis. Pathway enrichment analysis was used to explore functional differences among subpopulations characterize metabolic of cells. Results: comprehensively determined transcriptional landscape cells, particularly their transcriptomes during On basis molecular properties, we also types associated with transplantation-associated processes, including cytotoxic CD8 + activated conventional CD4 dysfunctional Tregs. further elucidated contribution JunB induction Th17 differentiation restriction Treg development, that HIF-1a participates metabolism function. Conclusions: present first systematic variation within population, providing new insights for development novel therapeutic targets rejection.